Press Releases | January 25, 2021

A research team at the Department of Mechanical Engineering at Toyohashi University of Technology developed a nanosecond pulse laser-assisted photoporation method using titanium-oxide nanotubes (TNTs) for highly efficient and low-cost intracellular delivery. The proof of concept for the possibility of intracellular delivery after irradiation with nanosecond pulse laser on TNTs was validated. TNTs were formed on titanium sheets using the electrochemical anodization technique at different voltages and times. HeLa - human cervical cancer cells were cultured in the nanotubes and submerged in a solution of biomolecules. After cells were exposed to nanosecond pulse laser, we successfully delivered propidium iodide (PI) and fluorescent dextran into cells with high efficiency and cell viability.

Full text: Titanium oxide nanotubes facilitate low-cost laser-assisted photoporation
TUT website: Press release