Kurita, Hirofumi
| Affiliation | Department of Applied chemistry and Life Science |
|---|---|
| Title | Associate Professor |
| Fields of Research | Applied Electrostatics, Application of Atmospheric Pressure Plasma, Cellular and Molecular Bioengineering |
| Degree | Ph.D. in Eng. (Toyohashi University of Technology) |
| Academic Societies | The Japan Society of Applied Physics, The Molecular Biology Society of Japan, The Institute of Electrostatics Japan, The Japan Society of Plasma Science and Nuclear Fusion Research, Society for Free Radical Research JAPAN, The Biophysical Society of Japa |
| kurita@chem Please append ".tut.ac.jp" to the end of the address above. |
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| Researcher information URL（researchmap） | Researcher information |
Research
Analysis of damage to biomacromolecules and cellular responses induced by atmospheric pressure plasma
Water-in-oil (W/O) droplet manipulation by using electrostatic force and its application for life science
Single-molecule DNA manipulation and measurement
Theme1：Damage to biomacromolecules and cellular responses induced by atmospheric pressure plasma
Overview
Biological and medical applications of non-thermal plasma (NTP) have been widely studied. In particular, an atmospheric pressure plasma jet (APPJ) is widely used because it can treat subjects without thermal loading, and length of the plasma jet can be adjusted by flow condition of noble gas. It is generally considered that reactive oxygen and nitrogen species (RONS) play an important role in plasma medicine due to a higher oxidation potential. To elucidate the cellular responses induced by exposure to NTP, we focused on (1) identification and quantification of reactive chemical species in aqueous media, (2) damage to biomacromolecules, and (3) cellular response of mammalian cells.
Selected publications and works
◆ H. Kurita, S. Miyachika, H. Yasuda, K. Takashima, and A. Mizuno, "Use of molecular beacons for the rapid analysis of DNA damage induced by exposure to an atmospheric pressure plasma jet," Applied Physics Letters, vol. 107, 263702 (5 pp.), Dec. 2015.
◆ E. Szili, N. Gaur, S.-H. Hong, H. Kurita, J.-S. Oh, M. Ito, A. Mizuno, A. Hatta, A. Cowin, D. Graves, and R. Short, "The assessment of cold atmospheric plasma treatment of DNA in synthetic models of tissue fluid, tissue and cells," Journal of Physics D: Applied Physics, vol. 50, 274001, July 2017.
◆ H. Kurita, N. Haruta, Y. Uchihashi, T. Seto, and K. Takashima, "Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation," PLOS ONE, vol. 15, e0232724, May 2020.
◆ S. Arai, K. Bidbayasakh, A. Fukuda, K. Takashima, and H. Kurita, "Oxidative modification in nuclear and mitochondrial DNA and its removal in A549 human lung cancer cells exposed to cold atmospheric-pressure plasma," Japanese Journal of Applied Physics, Vol. 61, 096003 (8 pp.), Sept. 2022.
Keywords
Theme2：Delivery of cell-impermeable molecules into cells using a water droplet in dielectric oil and electric field
Overview
We have developed a novel methodology for delivering exogenous DNA molecules into cells, using a water droplet in dielectric oil and DC electric field. We are investigating the mechanism of the gene transfer process and its application.
Selected publications and works
◆ Y. Tsurusaki, et al., PLOS ONE, vol. 18, e0285444 (16 pp.), May 2023.[PLOS]
◆ Y. Watanabe, et al., Sensors, Vol. 22, 2494 (13 pp.), Mar. 2022.[MDPI]
◆ H. Kurita, et al., PLOS ONE, Vol. 15, e0243361 (18 pp.), Dec. 2020.[PLOS]
◆ T. Ishino et al., Journal of Veterinary Medical Science, Vol. 82, pp. 14-22, Jan. 2020.[J-STAGE]
◆ H. Kurita, et al., Biochemistry and Biophysics Reports, vol. 8, pp. 81-88, Dec. 2016.[ScienceDirect]
◆ H. Kurita, et al., PLOS ONE, vol. 10, e0144254 (15 pp.), Dec. 2015.[PLOS]
◆ A. Asada, et al., IEEE Transactions on Industry Applications, vol. 49, pp. 311-315, Jan. 2013.[IEEE Xplore]
Keywords
Theme3：Single-molecule DNA manipulation and measurement
Overview
Recently, single-molecule imaging and single-molecule detection techniques have been investigated for the analysis of various biochemical processes. For example, atomic force microscopy imaging can provide a three-dimensional surface profile with nanometer resolution. On the other hand, fluorescence microscopy is also a useful tool for visualizing biological macromolecules and various reactions. Real-time fluorescent observations are particularly helpful for understanding single-molecule phenomena since the positions of individual protein or DNA molecules can be directly detected by fluorescent labeling.
Selected publications and works
◆ H. Kurita, K. Torii, H. Yasuda, K. Takashima, S. Katsura, and A. Mizuno, "The Effect of Physical Form of DNA on ExonucleaseIII Activity Revealed by Single-molecule Observations, Journal of Fluorescence, vol. 19, pp. 33-40, Feb 2009.
◆ H. Kurita, T. Takata, H. Yasuda, K. Takashima, and A. Mizuno, "A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract, Chemical Physics Letters, vol. 493, pp. 165-169, June 2010.
◆ S. Takahashi, S. Motooka, T. Usui, S. Kawasaki, H. Miyata, H. Kurita, T. Mizuno, S.-i. Matsuura, A. Mizuno, M. Oshige, and S. Katsura, "Direct single-molecule observations of local denaturation of a DNA double helix under a negative supercoil state," Analytical Chemistry, vol. 87, pp. 3490–3497, 2015.
Keywords
Title of class
Laboratory Works in Chemistry and Life Science
Advanced Molecular and Cellular Bioengineering
Advanced Biotechnology 1