|Affiliation||Department of Applied chemistry and Life Science|
|Fields of Research||Applied Electrostatics, Application of Atmospheric Pressure Plasma, Molecular Biology, Bioengineering|
|Degree||Ph.D. in Eng. (Toyohashi University of Technology)|
|Academic Societies||The Japan Society of Applied Physics, The Molecular Biology Society of Japan, The Institute of Electrostatics Japan, The Japan Society of Plasma Science and Nuclear Fusion Research, Society for Free Radical Research JAPAN, The Biophysical Society of Japa|
Please append ".tut.ac.jp" to the end of the address above.
|Laboratory website URL||https://chem.tut.ac.jp/plasma/|
|Researcher information URL（researchmap）||Researcher information|
Analysis of damage to biomacromolecules and cellular responses induced by atmospheric pressure plasma
Water-in-oil (W/O) droplet manipulation by using electrostatic force and its application for life science
Single-molecule DNA manipulation and measurement
Theme1：Damage to biomacromolecules and cellular responses induced by atmospheric pressure plasma
Biological and medical applications of non-thermal plasma (NTP) have been widely studied. In particular, an atmospheric pressure plasma jet (APPJ) is widely used because it can treat subjects without thermal loading, and length of the plasma jet can be adjusted by flow condition of noble gas. It is generally considered that reactive oxygen and nitrogen species (RONS) play an important role in plasma medicine due to a higher oxidation potential. To elucidate the cellular responses induced by exposure to NTP, we focused on (1) identification and quantification of reactive chemical species in aqueous media, (2) damage to biomacromolecules, and (3) cellular response of mammalian cells.
Selected publications and works
◆ H. Kurita, S. Miyachika, H. Yasuda, K. Takashima, and A. Mizuno, "Use of molecular beacons for the rapid analysis of DNA damage induced by exposure to an atmospheric pressure plasma jet," Applied Physics Letters, vol. 107, 263702 (5 pp.), Dec 2015.
◆ H. Kurita, J. Miyamoto, S. Miyachika, Y. Uchihashi, H. Yasuda, K. Takashima, and A. Mizuno, "Production of reactive oxygen and nitrogen species in a cell culture medium exposed to an atmospheric pressure plasma jet," MRS Advances, vol. 2, pp. 987-993, May 2017.
◆ E. Szili, N. Gaur, S.-H. Hong, H. Kurita, J.-S. Oh, M. Ito, A. Mizuno, A. Hatta, A. Cowin, D. Graves, and R. Short, "The assessment of cold atmospheric plasma treatment of DNA in synthetic models of tissue fluid, tissue and cells," Journal of Physics D: Applied Physics, vol. 50, 274001, July 2017.
◆ H. Kurita, N. Haruta, Y. Uchihashi, T. Seto, and K. Takashima, "Strand breaks and chemical modification of intracellular DNA induced by cold atmospheric pressure plasma irradiation," PLoS ONE, vol. 15, e0232724, May 2020.
Theme2：Water-in-oil (W/O) droplet manipulation by using electrostatic force and its application for life science
In recent years, a miniaturized reaction system, such as water-in-oil (W/O) droplets, has attracted increasing attention in the field of bioengineering and micro total analysis systems. Many applications of droplet-based reactors have engendered the development of various manipulation techniques for droplet formation, transportation, and fusion. It is known that dielectrophoretic force is effective for micromanipulation. By using dielectrophoretic force, operation of droplets such as translational motion, rotation, and noncontact trapping is possible. Coulomb force can also be used in micromanipulation of molecules and dispersed particles. The charged particle in an electric field moves along the line of electric force. In addition, we have developed a novel gene transfection method, W/O droplet electroporation, using dielectric oil and a liquid droplet containing living cells and exogenous DNA.
Selected publications and works
◆ A. Asada, H. Aoki, H. Kurita, A. Antoniu, H. Yasuda, K. Takashima, and A. Mizuno, "A novel gene transformation technique using water-in-oil droplet in an electrostatic field, IEEE Transactions on Industry Applications, vol. 49, pp. 311-315, Jan 2013.
◆ H. Kurita, S. Takahashi, A. Asada, M. Matsuo, K. Kishikawa, A. Mizuno, and R. Numano, "Novel Parallelized Electroporation by Electrostatic Manipulation of a Water-in-oil Droplet as a Microreactor," PLoS ONE, vol. 10, e0144254, Dec 2015.
◆ H. Kurita, Y. Takao, K. Kishikawa, K. Takashima, R. Numano, and A. Mizuno, "Fundamental study on a gene transfection methodology for mammalian cells using water-in-oil droplet deformation in a DC electric field," Biochemistry and Biophysics Reports, vol. 8, pp. 81-88, Dec 2016.
◆ T. Ishino, H. Kurita, R. Kirisawa, Y. Shimamoto, R. Numano, and H. Kitamura, "Introduction of a plasmid and a protein into bovine and swine cells by water-in-oil droplet electroporation," The Journal of Veterinary Medical Science, vol. 82, pp. 14-22, Jan 2020.
Theme3：Single-molecule DNA manipulation and measurement
Recently, single-molecule imaging and single-molecule detection techniques have been investigated for the analysis of various biochemical processes. For example, atomic force microscopy imaging can provide a three-dimensional surface profile with nanometer resolution. On the other hand, fluorescence microscopy is also a useful tool for visualizing biological macromolecules and various reactions. Real-time fluorescent observations are particularly helpful for understanding single-molecule phenomena since the positions of individual protein or DNA molecules can be directly detected by fluorescent labeling.
Selected publications and works
◆ H. Kurita, K. Torii, H. Yasuda, K. Takashima, S. Katsura, and A. Mizuno, "The Effect of Physical Form of DNA on ExonucleaseIII Activity Revealed by Single-molecule Observations, Journal of Fluorescence, vol. 19, pp. 33-40, Feb 2009.
◆ H. Kurita, T. Takata, H. Yasuda, K. Takashima, and A. Mizuno, "A kinetic analysis of strand breaks on large DNA induced by cigarette smoke extract, Chemical Physics Letters, vol. 493, pp. 165-169, June 2010.
◆ S. Takahashi, S. Motooka, T. Usui, S. Kawasaki, H. Miyata, H. Kurita, T. Mizuno, S.-i. Matsuura, A. Mizuno, M. Oshige, and S. Katsura, "Direct single-molecule observations of local denaturation of a DNA double helix under a negative supercoil state," Analytical Chemistry, vol. 87, pp. 3490–3497, 2015.
Title of class
Laboratory Works in Chemistry and Life Science
Laboratory Work in Chemistry