豊橋技術科学大学

Search

Search

Eki, Toshihiko

Affiliation Department of Applied chemistry and Life Science
Title Professor
Fields of Research Molecular Genetics / Molecular Biology / Functional Genomics
Degree PhD in Pharmaceutical Sciences (The University of Tokyo)
Academic Societies The Molecular Biology Society of Japan / The Pharmaceutical Society of Japan / The Japanese Biochemical Society / The Genetic Society of America
E-mail eki@chem
Please append ".tut.ac.jp" to the end of the address above.
Researcher information URL(researchmap) Researcher information

Research

環境問題に対する社会的な関心が高まり、人間と環境との関係が改めて見直されるようになっています。 地球上の生物は、苛酷な環境からのストレスに適応しながら子孫を増やす仕組みを獲得しています。なかでも遺伝物質であるゲノムDNAを維持する分子機構は生命の生存にとってとりわけ重要であり、ゲノム安定化の破綻は癌や遺伝病、不妊の原因になることも明らかになっています。そのため、生物の持つゲノム安定化の仕組みの理解、および遺伝子リスクの検知は重要な課題と考えられます。さらに、先端的な農業の推進には、土壌環境や土壌生物多様性を評価できる新技術の開発も重要です。私は以下の3つのテーマを掲げて、酵母や線虫などのモデル生物を用い、遺伝子工学や遺伝子機能解析法を駆使した研究を行っています。
(1)ゲノム安定性維持に関わる新規RNA干渉遺伝子群の研究

(2)合成生物学に基づくデザイナー酵母の創出

(3)DNAメタバーコード解析による土壌生物相分析法の開発と応用

Theme1:Studies on a novel RNAi gene drh-3 involved in maintenance of genome integrity in C. elegans

Overview

We identified a novel nematode gene drh-3 that functions in both maintenance of chromosome integrity and RNA interference (RNAi). Since the relationship between RNAi and chromosome regulations has been largely unknown, we have focused on functional analyses of DRH-3 protein in C. elegans. This study will contribute to progress in basic biomedical sciences.

Selected publications and works

Recent publications:
◆Toshihiko Eki., Takeshi Ishihara, Isao Katsura, and Fumio Hanaoka: A genome-wide survey and systematic RNAi-based characterization of helicase-like genes in Caenorhabditis elegans. DNA Res., 14(4), 183-199 (2007)
◆Masaharu Nakamura, Rumi Ando, Taro Nakazawa, Takuro Yudazono, Naoko Tsutsumi, Naoki Hatanaka, Toshiyasu Ohgake, Fumio Hanaoka, Toshihiko Eki: Dicer-related drh-3 gene functions in germ line development by maintenance of chromosomal integrity in Caenorhabditis elegans. Genes Cells, 12(9), 997-1010 (2007)
◆Toshihiko Eki and Fumio Hanaoka: Discovery of orphan helicases and deorphanization by genome-wide analyses in two model organisms, S. cerevisiae and C. elegans, Bacterial DNA, DNA polymerase and DNA Helicases. pp.411-432, Nova Science Publishers (2010)

Keywords

RNAi, C. elegans, genome integrity, drh-3 gene

Theme2:Development of genetically altered yeasts by synthetic biological approach

Overview

It is important to detect genome toxicities of chemicals and waste products. We have developed the biosensing assays by recombinant reporter yeasts for detecting genotoxicity. We also tried to develop recombinant yeasts for sensitive detection of genotoxicity by using the CRISPR/Cas9-mediated genome editing technology.

Selected publications and works

Recent publications:
◆Kohei Ichikawa and Toshihiko Eki: A novel yeast-based reporter assay system for the sensitive detection of genotoxic agents mediated by a DNA damage-inducible LexA-GAL4 protein. The Journal of Biochemistry (Tokyo), 139(1), 105-112 (2006)
◆Yukari Ochi, Harumi Sugawara, Mio Iwami, Megumi Tanaka and Toshihiko Eki: Sensitive detection of chemical-induced genotoxicity by the Cypridina secretory luciferase reporter assay using DNA repair-deficient strains of Saccharomyces cerevisiae. Yeast, 35(1), 265-278 (2011)
◆Hajime Suzuki, Takahiro Sakabe, Yuu Hirose, and Toshihiko Eki: Development and evaluation of yeast-based GFP and luciferase reporter assays for chemical-induced genotoxicity and oxidative damage. Applied Microbiology and Biotechnology, 101(2), 659-671 (2017)
◆Toshihiko Eki: Yeast-based genotoxicity tests for assessing DNA alterations and DNA stress responses: a 40-year overview. Applied Microbiology and Biotechnology, 102(6), 2493-2507 (2018)

Keywords

recombinant yeast, CRISPR/Cas9, genome editing, reporter, genotoxicity

Theme3:DNA metabarcode analysis of soil organisms for assessment of soil environment

Overview

We are developing novel DNA barcoding technique of soil organisms, especially soil nematodes using the next-generation sequencer for evaluating soil environment. We developed a novel lifespan-based assay for detecting biotoxicity of materials. This test will be useful for risk management of wasted chemicals because nematodes including C. elegans are majority of the living organisms in soil.

Selected publications and works

Recent publications:
◆Hiroaki Harada, Masaru Kurauchi, Rie Hayashi, and Toshihiko Eki: Shortened lifespan of nematode Caenorhabditis elegans after prolonged exposure to heavy metals and detergents. Ecotoxicology and Environmental Safety, 66(3), 378-383 (2007)
◆Masaru Kurauchi, Hisashi Morise, and Toshihiko Eki: Using the nematode Caenorhabditis elegans daf-16 mutant to assess the lifespan toxicity of prolonged exposure to ecotoxic agents. Journal of Health Science, 55(5), 796-804 (2009)
◆Toshihiko Eki:Ecotoxicology of free-living nematode Caenorhabiditis elegans, Ecotoxicology Around the Grobe, pp.1-52, Nova Science Publishers (2011)
◆Hisashi Morise, Erika Miyazaki, Shoko Yoshimitsu, and Toshihiko Eki: Profiling nematode communities in unmanaged flowerbed and agricultural field soils in Japan by DNA barcode sequencing. PLoS One, 7(12), e51785 (10 pages) (2012)

Keywords

nematode, soil biodiversity, agriculture, DNA barcoding, next-generation sequencer

to Pagetop