Research highlights

Biomedical applications of plasma technology

Atmospheric plasma is widely used for medical and biological applications including sterilization, selective killing of tumor cells, gene transfection, and healing wounds.

Acidic and radical species generated by plasma first access a plasma membrane, the outermost layer of a cell. However, it is unclear how these plasma-induced species affect and/or permeate plasma membranes.

Here, Ryugo Tero, Yoshiyuki Suda and colleagues at Toyohashi University of Technology report the effects of atmospheric plasma irradiation on an artificial plasma membrane system.

A phopholipid bilayer membrane was prepared on a silicon wafer in an aqueous solution, and the atmospheric plasma was irradiated with a home-build dielectric barrier discharge (DBD) instrument.

Observation with a fluorescence microscope and an atomic force microscope revealed that pores on the order of 10 nm to 1 µm in size were formed in the lipid bilayer membrane after the plasma irradiation. Capturing these micropores in a fluid lipid membrane is a significant advantage of the artificial lipid membrane system, and quantitative analysis of the pores was achieved. The results indicate that the micropores act as paths for the non-selective leakage or transportation of solutes into and out of cells during the plasma-induced phenomenon such as sterilization and gene transfection.

The artificial plasma membrane system is valuable for studying the fundamental effects of plasma on biomolecules for establishing medical and biological applications of plasmas.

Reference:

Authors: Ryugo Tero^1,2, Yoshiyuki Suda³, Ryo Kato³, Hideto Tanoue³ and Hirofumi Takikawa³

Title of original paper: Plasma irradiation of artificial cell membrane system at solid-liquid interface.

Journal, volume, pages and year: Applied Physics Express 7, 077001 (2014) [Open Access].

Digital Object Identifier (DOI): 10.7567/APEX.7.077001

Affiliations: ¹Electronics-Inspired Interdisciplinary Research Institute, ²Department of Environmental and Life Sciences, ³Department of Electrical and Electronic Information Engineering, Toyohashi University of Technology

Department website: http://www.eiiris.tut.ac.jp/

Related topics will be presented at 2014 MRS Fall Meeting (Nov. 30 - Dec. 5, 2014, Boston MA, by Y. Suda: http://www.mrs.org/fall2014/), and the 7th International Symposium on Surface Science (Nov. 2-6, 2014, Matsue, Japan, by R. Tero: http://www.sssj.org/isss7/)