|Affiliation||Department of Environmental and Life Sciences|
|Fields of Research||Surface physical chemistry|
|Degree||Doctor of Science, Graduate School of Science, the University of Tokyo|
|Academic Societies||the American Chemical Society, the International Association of Colloid and Interface Scientists (IACIS), the Chemical Society of Japan, the Japan Society of Applied Physics, the Surface Science Society of Japan, ｔhe Biophysical Society of Japan|
Please append "tut.jp" to the end of the address above.
|Laboratory website URL||http://www.eiiris.tut.ac.jp/tero/|
"Structure and dynamics of supported lipid bilayer membranes"
Lipid bilayers are fundamental structures of cell membranes, and provide reaction fields to membrane proteins relating to the transportation of signal, materials and energy into and/or out of cells. Artificial lipid bilayers on solid materials, called supported lipid bilayers (SLBs), are interfacial system between biomembranes and solid devices thus expected to be a platform for investigating membrane reaction. My research target is SLB formation on silicon and oxide substrates, and observation of dynamic processes in and on SLBs (e.g. lipid diffusion, domain formation/dissipation, peptides and protein assemblies) by using fluorescence-microscope-based techniques and atomic force microscope.
Theme1：Effects of substrate properties on supported lipid bilayer (SLB) formation and phase separation
SLBs are formed by vesicle fusion method in my lab. Substrates soaked in a suspension of lipid vesicles (=liposomes) are spontaneously coated by SLB through the vesicle transformation processes from sphere to planar membrane e.g. adsorption, fusion, rupture and spreading. In these processes, interaction between vesicles and substrates are critical factor thus SLB formation highly depends on the substrate conditions, vesicle size and lipid component. Understanding how the substrate physical and chemical properties (structures, roughness, charges, chemical termination, etc) affects to the SLB formation process, and furthermore the two dimensional structures in SLB, is essential for fabricating functional model biomembrane systems.
Theme2：In situ observation of molecular diffusion in SLB
Lateral diffusion of lipid molecules in cell membranes is fundamental process of domain formation and membrane reactions. I use single molecule tracking (SMT) method to observe the lipid diffusion in SLBs in situ. In my experimental setup, SMT is achieved independent of the substrate transparency or refractive index, thus silicon, TiO2 and other oxide materials are available. SMT on nano-structured oxide surfaces are currently underway.
Theme3：Artificial lipid bilayer platform on graphene
Graphene is a single atomic sheet consisting of sp2 carbon. Its high electron mobility has been attracted researcher's interests, but recently various unique properties of graphene is becoming known. One of them is the unordinary fluorescence quenching function. Emission of fluorescence probes are quenched independent of the wave length, and the efficient distance is much further than the conventional FRET between molecules. We are developing lipid bilayer platform on graphene and graphene oxide to develop a new method for lipids and proteins in and on lipid bilayer membranes.
Title of class
Cutting-Edge Interdisciplinary Research 3
Topics in Life and Materials Science
Seminar on Interdisciplinary Research